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POTENTIAL ROLE OF MESENCHYMAL STEM CELLS IN TREATMENT OF STZ DIABETIC INDUCED RAT MODEL REFERRING TO HEPATORENAL PROTECTIVE EFFECTS

Ahmad K Hassan*, Ibrahim El Zaatary, Sherifa Hamed and Ossam A Abbas

Diabetes is a complex chronic endocrinal distinguished with increased blood glucose level and insufficient insulin secretion and function. This disease is leading to many complications and considered as one of the most massive causes of mortality or morbidity around the world. The successful treatment of diabetes is how to regenerate pancreatic islets β-cells. The current study aimed to investigate the effect of mesenchymal stem cells (MSCs) transplantation as a new strategy for treatment of diabetes in Streptozotacin (STZ) induced diabetic male albino rats. To achieve this goal, thirty male albino rats (160-180 grams) were used in this work. Animals were divided into three groups; each of ten rats. Group I, Normal healthy control. Group II, STZ (45 mg/kg) induced diabetic group which left without treatment. Group III, STZ diabetic induced group and treated with MSC.s (2 × 106 cells/rat) via penial vein. After six weeks of treatment, animals were sacrificed; blood samples for biochemical estimations and organs of pancreas, liver, and kidney were removed for histopathological examination. The results revealed that the diabetic group had significant increase in glucose, ALT, AST, ALP, MDA, creatinine, urea, LDL, TGs, and cholesterol levels in addition to significant decrease in insulin and HDL levels. After six weeks of MSCs transplantation, the treated group showed a significant amelioration in these biochemical parameters. Histopathological examination showed an improvement in the histological structure of pancreas, liver and kidney tissues in the MSCs treated group compared with the degeneration observed in the diabetic untreated group which was in accordance with the biochemical finding. In conclusion: treatment with MSC.s transplantation as a cell-therapy could restore pancreatic β-cell function and improved liver and kidney functions in diabetes as a new strategy for diabetes treatment. However, more studies at this field are required.