Abstrato

Transient replication of human papillomavirus type 16 in HEK293T cells.

Amutikari Mamuti, Qian Shi, Tao Lv, Zhenghai Ma

Our knowledge regarding human papillomaviruses (HPVs) replication remains very limited, in part due to the lack of a cell culture system for HPV replication. Here we present evidence that the plasmid containing the full-length HPV-16 genome can replicate and produce virus-like particles (VLPs) in HEK293T cells. At 48 h after transfection of the plasmid containing HPV-16 genome into HEK293T cell line, HPV-16 VLPs were observed from the cells with an electron micrograph, the virus DNAs existed in HEK293 cells for at least 60 h, and the fresh HPV-16 DNAs were synthesized at 20 h after transfection. The transcripts of E1, L1 and E1^E4 also were detected by RT-PCR. Therefore, HPV-16 genes expressed and HPV-16 VLPs formed in the cells. These results suggest that HEK293T cells allow HPV-16 replicates transiently.