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In vitro experimental study of delayed intracranial hemorrhage due to vitamin K deficiency in mice infected with cytomegalovirus

Shan Cong, Wenjie Wang, Hongfeng Zhu, Weihong Kong

Objective: To investigate the changes of learning and memory function and neurological function in mice with intracranial hemorrhage caused by delayed vitamin K deficiency induced by cytomegalovirus infection.

Methods: Intracranial hemorrhage mice model (model group, n=10) that was infected with cytomegalovirus due to vitamin K deficiency was conducted. At the same time, the mice that had matched age and weight with those of the model group were selected as normal control group (n=10). Mice learning and memory performance was measured by Morris water maze test when these mice were raised up to the age of 2 months and compared between the two groups. And then the score of neurological test were observed and compared in the two groups.

Results: Compared with the normal control group, the average escape latency of model group mice was significantly prolonged. The times of crossing the platform of the mice in the model group decreased significantly. At the age of 2 months, the neurological function score of the model group was 2.75 ± 0.82, which was significantly higher than that of the control group.

Conclusion: The learning and memory ability of mice with intracranial hemorrhage caused by delayed vitamin K deficiency due to cytomegalovirus infection decreased. And there were different degrees of neurological deficits.