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Hypoxic microenvironment promotes proliferation and invasion of non-small cell lung cancer A549 Cells through Wnt/β-catenin signaling pathway

Ruidong Ma, Xiaoping He, Hong Wang, Weikun Jia, Xiaofei Zeng

Objective: To study the effect of Wnt/β-catenin signaling pathway on hypoxia induced proliferation and invasion in lung cancer cell line A549.

Methods: Cultured lung cancer A549 cells were treated with hypoxia for 24 h. The proliferation rates were detected by CCK8; the mRNA and protein expression levels of HIF-1α and β-catenin were detected by RT-PCR and Western blot; the effect of hypoxia on invasion ability of A549 cells were detected with Transwell method. Furthermore, β-catenin siRNA was used to investigate the role of β-catenin on hypoxia induced proliferation and invasion.

Results: The results demonstrated that hypoxia could promote A549 cells proliferation and invasion. The mRNA and protein level of HIF-1α and β-catenin was upregulated by hypoxia treatment, whereas β- catenin siRNA could block these processes.

Conclusion: Hypoxia promotes osteosarcoma cell line A549 proliferation and invasion by activating Wnt/β-catenin signaling pathway.