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Expression and significance of HLA-DR/CD14 in peripheral blood mononuclear cells of critical patients in ICU

Lijing Jia, Lili Wang, Yanan Wang, Heng Zhang, Da Ji, Wang Hong Xin, Wei Chen

Objective: To observe and explore the expression rate of HLA-DR/CD14 in peripheral blood monocytes of ICU patients and its clinical significance.

Methods: From October 2015 to April 2015 hospitalized patients with 60 cases, including ICU in critically ill patients (group A), 30 cases (group B) 30 patients with other diseases and 30 healthy physical examination to the same period as the healthy control group, the application of flow cytometry to detect the peripheral blood mononuclear cell surface the HLA-DR/CD14 expression rate, and the determination of research group A C-reactive protein (CRP) level, the analysis of CRP and HLA-DR/ CD14 expression rate.

Results: The study group A HLA-DR/CD14 expression rate (36.93+13.26)% was significantly lower than the group B (55.48 ± 20.38)% and healthy control group (75.84 ± 7.65)%, P<0.01 difference have statistical significance, and research group B was significantly lower than that of healthy controls (P<0.05). Correlation analysis showed that the expression rate of HLA-DR/CD14 in ICU patients was negatively correlated with CRP level (r=-0.689, P<0.01).

Conclusion: Peripheral blood mononuclear cell surface HLA-DR/CD14 expression rate is closely related to the severity of infection and disease, and can be used as a judgment of ICU critical illness severity and prognosis of immune index, worthy of clinical use.