Abstrato

Effect of Radiation on Hydroxyapatite-Chitosan-Gelatin-Agarose Scaffold for Bone Tissue Regeneration

Md. Masud Rana, Md. Arifuzzaman, Md. Shamim Miah, Naznin Akhtar, Sikder M Asaduzzaman

Development of tissue engineering scaffolds with native-like microarchitectures is an essential for generation of tissues. To overcome the bounds of traditional bone substitutes, hydroxyapatite and biodegradable polymer composites are taken attention for bone tissue regeneration. The main aim of this study was to fabricate a biomimetic scaffold through thermally induced phase separation technique, cross-linked with irradiation and evaluated their efficiency for bone tissue restoration. The fabricated scaffold was characterized by porosity, swelling kinetics, biodegradability, physicochemical interaction within the components and morphological properties. The porosity of the scaffold was measured by liquid displacement method, chemical structure was analyzed by fourier transform infrared analysis, morphological analysis was performed by light microscopic analysis etc. Among the fabricated scaffolds, cross linked with 25 kGy radiation doses scaffold was found to be more eligible for bone replacement due to its acceptable porosity, biodegradability and swelling ability. FTIR analysis also showed intermolecular interaction between components of the scaffold. Considering the light microscopic analysis of HCGA, it was found that the scaffold was porous and pores were interconnected for cell migration and regeneration.