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Combined effect of silver nanoparticles and honey in experimental wound healing process in rats

Guruvu Neeraja Rani, Bandaru Narasinga Rao, Shamili M, Jyothi Padmaja I

Background: Wounds are still major problem in developing countries, and may impose severe complications and high cost for therapy. Hence, wound management still remains an important focus of researches. Plastic catheters coated with SNPs prevent biofilm formation. Papaya possesses antibacterial activity which might prevent the multiplication of wound infection causing bacteria. The objective of this study was to combined effect of honey and silver nanoparticles increases the rate of healing in wounds infected by Pseudomonas aeruginosa.

Material and Methods: Eighteen healthy adult male Wistar albino rats weighing 250-300 g were used in this study. Excision wound model was used for this study. A wound of 500 mm2 was created using a sterile scalpel by removing the skin on the dorsum of the abdomen of the all group. Clinical assessments included observations concerning the appearance, and the wound size was measured.

Results: The wound in all the three groups were almost same in the beginning. The percentage of wound closure on the fifth day was 0.86 ± 0.11, 0.91 ± 0.18 and 0.95 ± 0.07, On d 10 still better wound closure was observed with a percentage there was a significant increase in healing and the percentage closure was 0.76 ± 0.20, 70.2 ± 0.19 and 71.15 ± 0.18 (P<0.001), on d 15 the wound closure percentage was 20.18 ± 0.18, 89.21 ± 0.18 and 94. 31 ± 0.20 (P<0.001), for control, honey treated and honey ± Ag Nps groups respectively with a high significance from control.

Conclusion: This study demonstrated the efficacy of multifloral honey mixed with silver nanoparticles in wound healing. Topical application of multifloral honey with silver nanoparticles accelerated the rate of wound healing by increasing the wound contraction compared with honey. The acceleration of wound healing rate and wound contraction through macroscopic and histological evolution on d 1, 5, 10 and 15. Hence, honey mixed with silver nanoparticles is potentially useful in dressing wounds.