Jornal de Medicina e Terapia Molecular


Characterization of glycine release from mouse hippocampal slices.

Simo S Oja, O Sakari Oja, Pirjo Saransaari

Glycine release from the mouse hippocampus was studied with a superfusion system in which freely floating slices were exposed to a continuous flow of medium. The slices were exposed to K+ stimulation and ischemic conditions in addition to a great number of synaptic effectors and second messengers. The release was markedly increased by K+ stimulation and under ischemic conditions. Many of the synaptic effectors and second messengers did not regulate the release, but nitric oxide was strongly stimulatory. The release was apparently inhibited by adenosine receptors and inhibition of phosphodiesterases.