Abstrato

Assessment of bone regeneration in mandibular defect with hyaluronic acid and rosemary to nanoparticles hydroxyapatite in rabbits.

Noor A Sulaiman*, Israa A Abdulhameed, Huda A Salim

This study was conducted to assess the effectiveness of a newly formulated novel paste of natural herbs in bone defect regeneration in rabbits. A total of 42 adult male rabbits were included in this study and randomized into three groups according to healing periods and bone defect induced. The bone defects were treated with different materials added to hydroxyapatite which is made from seashells which served as a control, these are Hyaluronic acid, Rosemary with Hyaluronic acid. At week one, the control group presented with an inflammatory reaction score of 2 that increased gradually to a mean of scoring 3.85 at 1 month, these results were also seen in granulation tissues and blood vessel formation. New bone, osteoblast, and osteoclast formation revealed the same results with a mean of 0.42 at 1 week and 1.42 in 2 weeks later, which increased to 2.85 at 1 month for both new bone formation and osteoblast, while osteoclast was 3 after 1 month. In a group treated with (hydroxyapatite and hyaluronic acid), the risk of inflammatory reactions and blood vessel formation increased, while decreasing in means of granulation tissue formation than the control group during the study period, both new bone and osteoblast formation showed the same mean with 1.42 at 1 week, and 2.14 at 2 weeks then increased to 3 after 1 month, there were no osteoclast cells present. The last group showed an inflammatory reaction mean of 4 through the study period, granulation tissue formation mean was 2 that increased to 3; blood vessels means was 3 that decreased to 1 after 1 month, new bone and osteoblast formation revealed similar results with a mean of 3 with no osteoclast cells. Rosemary has an anti-inflammatory effect and can accelerate bone formation by increasing the differentiation of mesenchymal cells to osteoblast cells.